Factors regulating production of <b>α</b> ‐galactosidase from <b> <i>Bacillus</i> </b> sp. JF <sub> <b>2</b> </sub>
X. Li(Horological Research Institute of Light Industry), L. Yang(Horological Research Institute of Light Industry), Panpan Yan(Horological Research Institute of Light Industry), Fanglei Zuo(Horological Research Institute of Light Industry), Jin Feng-xie(Horological Research Institute of Light Industry)
Cited by 14
Abstract
Certain factors affecting the production of cell‐associated α‐galactosidase by Bacillus sp. JF2 were investigated. The intention was to maximize α‐galactosidase activity of potential commercial application, by consecutive optimization of growth media and conditions. The highest α‐galactosidase activity was obtained when grown on melibiose, whereas sucrose inhibited the production of α‐galactosidase. α‐Galactosidase production was optimally active at pH 7·5 and 55°C. It was identified that a soy effluent stream could be used as the best carbon source for α‐galactosidase by Bacillus sp. JF2.
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