PCR AMPLIFICATION ON WHOLE BLOOD SAMPLES TREATED WITH DIFFERENT COMMONLY USED ANTICOAGULANTS

Vladimir Djordjević(Institute of Molecular Genetics and Genetic Engineering), Marija Stanković(Institute of Molecular Genetics and Genetic Engineering), Aleksandra Nikolić(Institute of Molecular Genetics and Genetic Engineering), Nebojša Antonijević(Institute for Cardiovascular Diseases of Vojvodina), L.J. Rakicevic(Institute of Molecular Genetics and Genetic Engineering), Aleksandra Divac Rankov(Institute of Molecular Genetics and Genetic Engineering), Milica Radojković(University Hospital Center Dr Dragiša Mišović)
Pediatric Hematology and Oncology
January 1, 2006
10.1080/08880010600751900
Cited by 19

Abstract

The aim of this study was to explore the influence of different anticoagulants on PCR amplification, as well as to optimize PCR amplification of blood samples for successful mutation detection. For the purpose of this study the amplification of part of methylenetetrahydrofolate reductase (MTHFR) gene exon 4, in which mutation C677T is located, was performed. With the exception of sodium heparin, the presence of other commonly used anticoagulants (sodium citrate, K3EDTA, lithium heparin) made it possible to obtain satisfactory amplification. The described method, apart from saving time and material, enables successful PCR-based analysis even when a very small amount of blood sample is available.

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