Fluorogenic Phospholipid Substrate to Detect Lysophospholipase D/Autotaxin Activity
Colin G. Ferguson(University of Utah), Cleve S. Bigman(Echelon Biosciences), Robyn D. Richardson(University of Utah), Laurens A. van Meeteren(Echelon Biosciences), Wouter H. Moolenaar(University of Utah), Glenn D. Prestwich(Echelon Biosciences)
Cited by 109Open Access
Abstract
[reaction: see text] Lysophospholipase D (lysoPLD), also known as autotaxin (ATX), is an important source of the potent mitogen lysophosphatidic acid (LPA). Two fluorogenic substrate analogues for lysoPLD were synthesized in nine steps from (S)-PMB-glycerol. The substrates (FS-2 and FS-3) show significant increases in fluorescence when treated with recombinant ATX and have potential applications in screening for this emerging drug target.
Related Papers
No related papers found
Powered by citation graph analysis