Optimized speckle variance OCT imaging of microvasculature
Adrian Mariampillai(University of Toronto), Michael K. K. Leung(University of Toronto), Mark T. Jarvi(University of Toronto), Beau A. Standish(Toronto Metropolitan University), Kenneth Lee(University of Toronto), Brian C. Wilson(University of Toronto), I. Alex Vitkin(University of Toronto), Victor X. D. Yang(Toronto Metropolitan University)
Cited by 273
Abstract
We optimize speckle variance optical coherence tomography (svOCT) imaging of microvasculature in high and low bulk tissue motion scenarios. To achieve a significant level of image contrast, frame rates must be optimized such that tissue displacement between frames is less than the beam radius. We demonstrate that higher accuracy estimates of speckle variance can enhance the detection of capillaries. These findings are illustrated in vivo by imaging the dorsal window chamber model (low bulk motion). We also show svOCT imaging of the nonstabilized finger (high bulk motion), using optimized imaging parameters, demonstrating better vessel detection than Doppler OCT.