Requirement for V <sub>α</sub> 14 NKT Cells in IL-12-Mediated Rejection of Tumors
Junqing Cui(Japan Science and Technology Agency), Tahiro Shin(Japan Science and Technology Agency), Tetsu Kawano(Japan Science and Technology Agency), Hiroshi Sato(Japan Science and Technology Agency), Eisuke Kondo(Japan Science and Technology Agency), Isao Toura(Japan Science and Technology Agency), Yoshikatsu Kaneko(Japan Science and Technology Agency), Haruhiko Koseki(Japan Science and Technology Agency), Masamoto Kanno(Japan Science and Technology Agency), Masaru Taniguchi(Japan Science and Technology Agency)
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Abstract
A lymphocyte subpopulation, the Valpha14 natural killer T (NKT) cells, expresses both NK1.1 and a single invariant T cell receptor encoded by the Valpha14 and Jalpha281 gene segments. Mice with a deletion of the Jalpha281 gene segment were found to exclusively lack this subpopulation. The Valpha14 NKT cell-deficient mice could no longer mediate the interleukin-12 (IL-12)-induced rejection of tumors. Although the antitumor effect of IL-12 was thought to be mediated through natural killer cells and T cells, Valpha14 NKT cells were found to be an essential target of IL-12, and they mediated their cytotoxicity by an NK-like effector mechanism after activation with IL-12.
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