Analysis of expressed sequence tags from <it>Actinidia</it>: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening

Richardson Annette C(Plant & Food Research), Rassam Maysoon(Plant & Food Research), McNeilage Mark A(Plant & Food Research), Nain Bhawana(Plant & Food Research), MacDiarmid Robin M(Plant & Food Research), Lesley L. Beuning(Plant & Food Research), Klages Karin(Plant & Food Research), Janssen Bart J(Plant & Food Research), Hellens Roger P(Plant & Food Research), Gera Emma(Plant & Food Research), Fraser Lena G(Plant & Food Research), Ferguson A Ross(Plant & Food Research), Eckloff Rheinhart(Plant & Food Research), Davy Marcus W(Max Planck Society), Bowen Judith H(Plant & Food Research), Helen Boldingh(Plant & Food Research), Allan Andrew C(Plant & Food Research), Usadel Björn(Plant & Food Research), Schaffer Robert J(Plant & Food Research), Newcomb Richard D(Plant & Food Research), Montefiori Mirco(Plant & Food Research), Matich Adam J(Plant & Food Research), Marsh Ken B(Plant & Food Research), Chagne David(Plant & Food Research), Bulley Sean M(Plant & Food Research), Kim Lo(Plant & Food Research), Atkinson Ross G(Plant & Food Research), Ampomah-Dwamena Charles(Plant & Food Research), M.A. McNeilage(Plant & Food Research), Gleave Andrew P(Plant & Food Research), Crowhurst Ross N(Plant & Food Research), Erik H. A. Rikkerink(Plant & Food Research), Gavin S. Ross(Plant & Food Research), Roswitha Schröder(Plant & Food Research), Kimberley C. Snowden(Plant & Food Research), Edwige Souleyre(Plant & Food Research), Matt D Templeton(Plant & Food Research), Eric F. Walton(Plant & Food Research), Daisy Wang(Plant & Food Research), Mindy Wang(Plant & Food Research), Yanming Y Wang(Plant & Food Research), Marion Wood(Plant & Food Research), Rongmei Wu(Plant & Food Research), Yar‐Khing Yauk(Plant & Food Research), William A. Laing(Plant & Food Research)
DOAJ (DOAJ: Directory of Open Access Journals)
July 1, 2008
10.1186/1471-2164-9-351
Cited by 212Open Access
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Abstract

Abstract Background Kiwifruit (Actinidia spp.) are a relatively new, but economically important crop grown in many different parts of the world. Commercial success is driven by the development of new cultivars with novel consumer traits including flavor, appearance, healthful components and convenience. To increase our understanding of the genetic diversity and gene-based control of these key traits in Actinidia, we have produced a collection of 132,577 expressed sequence tags (ESTs). Results The ESTs were derived mainly from four <it>Actinidia </it>species (<it>A. chinensis, A. deliciosa, A. arguta </it>and <it>A. eriantha</it>) and fell into 41,858 non redundant clusters (18,070 tentative consensus sequences and 23,788 EST singletons). Analysis of flavor and fragrance-related gene families (acyltransferases and carboxylesterases) and pathways (terpenoid biosynthesis) is presented in comparison with a chemical analysis of the compounds present in <it>Actinidia </it>including esters, acids, alcohols and terpenes. ESTs are identified for most genes in color pathways controlling chlorophyll degradation and carotenoid biosynthesis. In the health area, data are presented on the ESTs involved in ascorbic acid and quinic acid biosynthesis showing not only that genes for many of the steps in these pathways are represented in the database, but that genes encoding some critical steps are absent. In the convenience area, genes related to different stages of fruit softening are identified. Conclusion This large EST resource will allow researchers to undertake the tremendous challenge of understanding the molecular basis of genetic diversity in the <it>Actinidia </it>genus as well as provide an EST resource for comparative fruit genomics. The various bioinformatics analyses we have undertaken demonstrates the extent of coverage of ESTs for genes encoding different biochemical pathways in <it>Actinidia</it>.

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