High-throughput screening of catalytically inactive mutants of protein tyrosine phosphatases (PTPs) in a phosphopeptide microarray

Hongyan Sun(National University of Singapore), Lay Pheng Tan(National University of Singapore), Liqian Gao(National University of Singapore), Shao Q. Yao(National University of Singapore)
Chemical Communications
December 12, 2008
Cited by 50

Abstract

We report herein a novel phosphopeptide microarray capable of noncovalently "trapping" catalytically inactive mutants of protein tyrosine phosphatases (PTPs), and its application in high-throughput determination of PTP substrate specificity.


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