HPLC Enantioseparation Of Dl-and Tripeptides on Cyclodextrin Bonded Stationary Phases After Derivatization with 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate (AQC)

Abstract

Abstract Enantiomeric separations were performed on a number of di- and tripeptides after their pre-column derivatization with the fluorescence derizatizing agent 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). It has been demonstrated that the choice of a suitable cyclodextrin bonded phase in conjunction with nonaqueous polar mobile phases offers an effective means to resolve chiral peptides. It was found that the strength of interaction between the cyclodextrin and the solute, and therefore the retention and stereoselectivity, is extremely sensitive to small structural variations of the analyte.