Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins

Benjamin Wilhelm(Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103), Sunit Mandad(Max Planck Institute for Biophysical Chemistry), Sven Truckenbrodt(Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103), Katharina Kröhnert(Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103), Christina Schäfer(Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103), Burkhard Rammner(Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103), Seong Joo Koo(Leibniz-Forschungsinstitut für Molekulare Pharmakologie), Gala A. Claßen(Leibniz-Forschungsinstitut für Molekulare Pharmakologie), M. Krauß(Leibniz-Forschungsinstitut für Molekulare Pharmakologie), Volker Haucke(Leibniz-Forschungsinstitut für Molekulare Pharmakologie), Henning Urlaub(Universitätsmedizin Göttingen), Silvio O. Rizzoli(Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103)
Science
May 29, 2014
10.1126/science.1252884
Cited by 811

Abstract

Synaptic vesicle recycling has long served as a model for the general mechanisms of cellular trafficking. We used an integrative approach, combining quantitative immunoblotting and mass spectrometry to determine protein numbers; electron microscopy to measure organelle numbers, sizes, and positions; and super-resolution fluorescence microscopy to localize the proteins. Using these data, we generated a three-dimensional model of an "average" synapse, displaying 300,000 proteins in atomic detail. The copy numbers of proteins involved in the same step of synaptic vesicle recycling correlated closely. In contrast, copy numbers varied over more than three orders of magnitude between steps, from about 150 copies for the endosomal fusion proteins to more than 20,000 for the exocytotic ones.

Related Papers

No related papers found

Powered by citation graph analysis