DNA Binding Activity of Recombinant SRY from Normal Males and XY Females
Vincent R. Harley(The Honourable Society of Lincoln's Inn), David I. Jackson(The Honourable Society of Lincoln's Inn), Patrick Hextall(The Honourable Society of Lincoln's Inn), J. Ross Hawkins(The Honourable Society of Lincoln's Inn), Gary D. Berkovitz(Johns Hopkins University), Shanthini Sockanathan(Medical Research Council), Robin Lovell‐Badge(Medical Research Council), Peter N. Goodfellow(The Honourable Society of Lincoln's Inn)
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Abstract
The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence AACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.
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