Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor

Linda Park, Unja Martin, Kirsten E. Garka, Brian Gliniak, James P. Di Santo(Institut Pasteur), Werner Müller, David A. Largaespada(National Cancer Institute), Neal G. Copeland(National Cancer Institute), Nancy A. Jenkins(National Cancer Institute), Andrew G. Farr(University of Washington), Steven F. Ziegler(Virginia Mason Medical Center), Philip Morrissey, Raymond J. Paxton, John E. Sims
The Journal of Experimental Medicine
August 28, 2000
10.1084/jem.192.5.659
Cited by 414Open Access
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Abstract

The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Ralpha chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Ralpha(-/)- mice, but did activate cells from gammac(-/)- mice. Thus, the functional TSLPR requires the IL-7Ralpha chain, but not the gammac chain for signaling.

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