High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography
Sébastien Boutet(SLAC National Accelerator Laboratory), Lukas Lomb(Universität Hamburg), Garth J. Williams(SLAC National Accelerator Laboratory), Thomas R. M. Barends(Universität Hamburg), Andrew Aquila(Universität Hamburg), R. Bruce Doak(Arizona State University), Uwe Weierstall(Arizona State University), Daniel P. DePonte(Universität Hamburg), Jan Steinbrener(Universität Hamburg), Robert L. Shoeman(Universität Hamburg), M. Messerschmidt(SLAC National Accelerator Laboratory), Anton Barty(Universität Hamburg), Thomas A. White(Universität Hamburg), Stephan Kassemeyer(Universität Hamburg), Richard A. Kirian(Arizona State University), M. Seibert(SLAC National Accelerator Laboratory), P. Montanez(SLAC National Accelerator Laboratory), C. Kenney(SLAC National Accelerator Laboratory), Ryan Herbst(SLAC National Accelerator Laboratory), Philip Hart(SLAC National Accelerator Laboratory), J. Pines(SLAC National Accelerator Laboratory), G. Haller(SLAC National Accelerator Laboratory), Sol M. Grüner(Cornell University), Hugh T. Philipp(Cornell University), Mark W. Täte(Cornell University), Marianne S. Hromalik(State University of New York at Oswego), Lucas J. Koerner(Johns Hopkins University Applied Physics Laboratory), Niels van Bakel(National Institute for Subatomic Physics), John Morse(European Synchrotron Radiation Facility), W. Ghonsalves(SLAC National Accelerator Laboratory), David Arnlund(University of Gothenburg), Michael J. Bogan(SLAC National Accelerator Laboratory), Carl Caleman(Universität Hamburg), Raimund Fromme(Arizona State University), Christina Y. Hampton(SLAC National Accelerator Laboratory), Mark S. Hunter(Arizona State University), Linda C. Johansson(University of Gothenburg), Gergely Katona(University of Gothenburg), Christopher Kupitz(Arizona State University), Mengning Liang(Universität Hamburg), Andrew V. Martin(Universität Hamburg), Karol Nass(Universität Hamburg), Lars Redecke(Universität Hamburg), Francesco Stellato(Universität Hamburg), Nicuşor Tı̂mneanu(Uppsala University), Dingjie Wang(Arizona State University), Nadia A. Zatsepin(Arizona State University), Donald W. Schafer(SLAC National Accelerator Laboratory), James Defever(SLAC National Accelerator Laboratory), Richard Neutze(University of Gothenburg), Petra Fromme(Arizona State University), John C. H. Spence(Arizona State University), Henry N. Chapman(Universität Hamburg), Ilme Schlichting(Universität Hamburg)
Cited by 861Open Access
Abstract
Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.
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