Zinc Deficiency-induced Iron Accumulation, a Consequence of Alterations in Iron Regulatory Protein-binding Activity, Iron Transporters, and Iron Storage Proteins

Abstract

One consequence of zinc deficiency is an elevation in cell and tissue iron concentrations. To examine the mechanism(s) underlying this phenomenon, Swiss 3T3 cells were cultured in zinc-deficient (D, 0.5 μm zinc), zinc-supplemented (S, 50 μm zinc), or control (C, 4 μm zinc) media. After 24 h of culture, cells in the D group were characterized by a 50% decrease in intracellular zinc and a 35% increase in intracellular iron relative cells in the and increase in iron and and zinc iron of zinc-deficient cells of iron and of a in zinc-deficient cells an of of of zinc intracellular by the of the or the D the zinc deficiency in in iron and iron One consequence of zinc deficiency is an elevation in cell and tissue iron concentrations. To examine the mechanism(s) underlying this phenomenon, Swiss 3T3 cells were cultured in zinc-deficient (D, 0.5 μm zinc), zinc-supplemented (S, 50 μm zinc), or control (C, 4 μm zinc) media. After 24 h of culture, cells in the D group were characterized by a 50% decrease in intracellular zinc and a 35% increase in intracellular iron relative cells in the and increase in iron and and zinc iron of zinc-deficient cells of iron and of a in zinc-deficient cells an of of of zinc intracellular by the of the or the D the zinc deficiency in in iron and iron deficiency in and tissue of in the of and in a consequence of zinc in the of of a in zinc in the of iron in and in of zinc-deficient tissue iron is a of and is a iron is and iron by and of iron and a by iron is by the iron iron iron is the cell iron iron is in is or in an iron by a of and the iron or the of iron the the of iron in is by control of iron and or and control of is by iron and of the iron the of iron the of of iron is a of and of the iron in the of a the of and the of this a the iron the of or in of the of and by and by and and by and the of the is by iron the iron the in the of of the the iron the iron and the in by the an the and iron and iron of iron deficiency and iron of iron examine of the in iron and a iron zinc tissue iron a zinc deficiency a the of iron and and the and were and were were were and the were and and were and were and the and and a were by of and were were and and were by and were and were and were were were of and by of and were of by (D, 0.5 μm zinc) a the To the zinc the of and (S, 50 μm zinc) D by a of a of 50 μm D and by of (C, 4 μm zinc) and a zinc of 4 μm μm and a of 3T3 cells were and were in in were a of cells After 24 and cells were by in 24 h were and cells were the 24 and and and were by the of cell by and the cells were of the by and the cells were in a After the cells were and by and cell by a and of and the of of by 4 the of zinc and a of in were and of of cell and were the the were the by the of and were of were and of in 0.5 and were by were and by One the in a of and relative of and and the by of of were of of the in of in μm and μm in an of by and were and 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group relative the and in the increase in iron concentrations. of in a elevation in in the D group h the and in the D group relative the control is 24 h a consequence of the iron in the the of the in the D group the and the a zinc a control iron μm of 24 in the decrease in iron μm in 24 in of and 3T3 were cultured in or D the of of h of by μm and μm and 24 and of of in the the of a iron this is in intracellular is a the of cell or iron To examine the of were by the in h of culture, iron 24 h and in the D group relative the and the of 3T3 were cultured in or D the and were or is the of the of iron in the and in zinc-deficient 3T3 cells in a the of the of the iron 24 and the iron the D group relative the and the increase in in the of 3T3 were cultured in or D the of of and the and in by the of iron and zinc the increase in iron in the D 24 relative the and decrease in by of cell and in the of or were To the the decrease in a consequence of in zinc-deficient D or μm of control 24 h of 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this the the increase in is a consequence of zinc of of the iron in zinc-deficient 3T3 cells in this and the in a the increase in the D group is iron by and of the increase in zinc deficiency is and and the in in the and of the in the of zinc the of the increase in in an decrease in of the increase in of and zinc deficiency in 3T3 zinc deficiency the increase in in After in were a of and of the the in of the the is the or by or in cells or in cell the of of cells or cell and or this of in the D group by the the increase in by is the is in cells and cell or is in zinc-deficient cells and in is the in this of the in this of the is in zinc-deficient a zinc in and a zinc-deficient 3T3 cells characterized by an elevation in by and an of a a is and is of by the of the the of and the of the in 3T3 cells zinc deficiency of the the of this of D the of and iron in zinc-deficient cells by zinc the a a zinc the of iron in cells examine the relative of iron cells a consequence of zinc deficiency 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