Low density lipoprotein undergoes oxidative modification in vivo.

Wulf Palinski(University of California San Diego), Michael E. Rosenfeld(University of California San Diego), Seppo Ylä‐Herttuala(University of California San Diego), G.C. Gurtner(University of California San Diego), Steve S. Socher(University of California San Diego), S W Butler(University of California San Diego), Sampath Parthasarathy(University of California San Diego), T E Carew(University of California San Diego), Daniel Steinberg(University of California San Diego), J L Witztum(University of California San Diego)
Proceedings of the National Academy of Sciences
February 1, 1989
10.1073/pnas.86.4.1372
Cited by 1,525Open Access
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Abstract

It has been proposed that low density lipoprotein (LDL) must undergo oxidative modification before it can give rise to foam cells, the key component of the fatty streak lesion of atherosclerosis. Oxidation of LDL probably generates a broad spectrum of conjugates between fragments of oxidized fatty acids and apolipoprotein B. We now present three mutually supportive lines of evidence for oxidation of LDL in vivo: (i) Antibodies against oxidized LDL, malondialdehyde-lysine, or 4-hydroxynonenal-lysine recognize materials in the atherosclerotic lesions of LDL receptor-deficient rabbits; (ii) LDL gently extracted from lesions of these rabbits is recognized by an antiserum against malondialdehyde-conjugated LDL; (iii) autoantibodies against malondialdehyde-LDL (titers from 512 to greater than 4096) can be demonstrated in rabbit and human sera.

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