Comparative Genomic Hybridization for Molecular Cytogenetic Analysis of Solid Tumors
Anne Kallioniemi(University of California, San Francisco), Olli Kallioniemi(University of California, San Francisco), Damir Sudar(University of California, San Francisco), Denis Rutovitz(Western General Hospital), Joe W. Gray(University of California, San Francisco), Fred Waldman(University of California, San Francisco), Dan Pinkel(University of California, San Francisco)
Cited by 3,314
Abstract
Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.