The Transgenic RNAi Project at Harvard Medical School: Resources and Validation

Lizabeth A. Perkins(Harvard University), Laura Holderbaum(Harvard University), Rong Tao(Harvard University), Yanhui Hu(Harvard University), Richelle Sopko(Harvard University), Kim McCall(Boston University), Donghui Yang‐Zhou(Harvard University), I. R. Flockhart(Harvard University), Richard Binari(Howard Hughes Medical Institute), Hye-Seok Shim(Harvard University), Audrey Miller(Harvard University), Amy Housden(Harvard University), Marianna Foos(Harvard University), Sakara Randkelv(Harvard University), Colleen Kelley(Harvard University), Pema Namgyal(Harvard University), Christians Villalta(Howard Hughes Medical Institute), Lu-Ping Liu(Harvard University), Xia Jiang, Huanhuan Qiao, Xia Wang, Asao Fujiyama(National Institute of Genetics), Atsushi Toyoda(National Institute of Genetics), Kathleen Ayers(Yale University), Allison Blum(Howard Hughes Medical Institute), B. Czech(University of Cambridge), Ralph A. Neumüller(Harvard University), Dong Yan(Harvard University), Amanda Cavallaro(Howard Hughes Medical Institute), Karen L Hibbard(Howard Hughes Medical Institute), D Hall(Howard Hughes Medical Institute), Lynn Cooley(Yale University), Gregory J. Hannon(University of Cambridge), Ruth Lehmann(Howard Hughes Medical Institute), Annette L. Parks, Stephanie E. Mohr(Harvard University), Ryu Ueda(National Institute of Genetics), Shu Kondo(National Institute of Genetics), Jian-Quan Ni(Harvard University), Norbert Perrimon(Howard Hughes Medical Institute)
Genetics
August 27, 2015
10.1534/genetics.115.180208
Cited by 720Open Access
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Abstract

To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT-qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes. Data on the characterization of the lines either by RT-qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China).

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