Determination of Patulin in Apple Juice by Liquid Chromatography: Collaborative Study

Allan R. Brause; Mary W Trucksess; Frederick S Thomas; Samuel W Page; Collaborators:; J Burke; Amelia R. Tanner; S Hammack; BD Woodward; S Post; DG. Simmons; G Cherix; Michael Kennedy; Simon W. Lewis; Nicholas R. English; Elia D Coppola; L Kline; A Coopersmith; J. Foos; Thomas Eisele; Darcy A. Krueger; Collaborators:; H J Hofsommer; Susan MacDonald; F. Hesford; Scott Lea; P. Symonds; Gilles Martin; Jale Acar; A. Ekşi̇; R Ardenghi; Jerry Weiss; B. Lee; H. Woidich

doi:10.1093/jaoac/79.2.451

Determination of Patulin in Apple Juice by Liquid Chromatography: Collaborative Study

Allan R. Brause(Analytical Services), Mary W Trucksess(Center for Food Safety and Applied Nutrition), Frederick S Thomas(Center for Food Safety and Applied Nutrition), Samuel W Page(Center for Food Safety and Applied Nutrition), Collaborators:(Center for Food Safety and Applied Nutrition), J Burke(Center for Food Safety and Applied Nutrition), Amelia R. Tanner(Center for Food Safety and Applied Nutrition), S Hammack(Center for Food Safety and Applied Nutrition), BD Woodward(Center for Food Safety and Applied Nutrition), S Post(Center for Food Safety and Applied Nutrition), DG. Simmons(Center for Food Safety and Applied Nutrition), G Cherix(Center for Food Safety and Applied Nutrition), Michael Kennedy(Center for Food Safety and Applied Nutrition), Simon W. Lewis(Analytical Services), Nicholas R. English(Center for Food Safety and Applied Nutrition), Elia D Coppola(Center for Food Safety and Applied Nutrition), L Kline(Center for Food Safety and Applied Nutrition), A Coopersmith(Center for Food Safety and Applied Nutrition), J. Foos(Center for Food Safety and Applied Nutrition), Thomas Eisele(Center for Food Safety and Applied Nutrition), Darcy A. Krueger(Center for Food Safety and Applied Nutrition), Collaborators:(Center for Food Safety and Applied Nutrition), H J Hofsommer(Center for Food Safety and Applied Nutrition), Susan MacDonald(Center for Food Safety and Applied Nutrition), F. Hesford(Center for Food Safety and Applied Nutrition), Scott Lea(Center for Food Safety and Applied Nutrition), P. Symonds(Center for Food Safety and Applied Nutrition), Gilles Martin(Center for Food Safety and Applied Nutrition), Jale Acar(Center for Food Safety and Applied Nutrition), A. Ekşi̇(Center for Food Safety and Applied Nutrition), R Ardenghi(Analytical Services), Jerry Weiss(Center for Food Safety and Applied Nutrition), B. Lee, H. Woidich

Journal of AOAC International

March 1, 1996

10.1093/jaoac/79.2.451

Cited by 91Open Access

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Abstract

An AOAC International-International Union of Pure and Applied Chemistry-International Fruit Juice Union (AOAC-IUPAC-IFJU) collaborative study was conducted to evaluate a liquid chromatographic (LC) procedure for determination of patulin in apple juice. Patulin is a mold metabolite found naturally in rotting apples. Patulin is extracted with ethyl acetate, treated with sodium carbonate solution, and determined by reversed-phase LC with UV detection at 254 or 276 nm. Water, water-tetrahydrofuran, or water-acetonitrile was used as mobile phase. Levels determined in spiked test samples were 20, 50, 100, and 200 micrograms/L. A test sample naturally contaminated at 31 micrograms/L was also included. Twenty-two collaborators in 10 countries analyzed 12 test samples of apple juice. Recoveries averaged 96%, with a range of 91-108%. Repeatability relative standard deviations (RSDr) ranged from 10.9 to 53.8%. The reproducibility relative standard deviation (RSDR) ranged from 15.1 to 68.8%. The LC method for determination of patulin in apple juice has been adopted first action by AOAC INTERNATIONAL.

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