Studies on the nutrition of rice cell culture I. A simple, defined medium for rapid growth in suspension culture

Abstract

We investigated the nutrient requirements of rice in liquid culture and developed a revised medium of mineral salts, sucrose, thiamine and 2,4-dichlorophenoxyacetic acid. The following major nutrients at the indicated concentrations were beneficial: NO3-N (40 mM), NH4-N (5.0 mM), P (2.0 mM) and K (40 mM). Cobalt, iodine, pyridoxine, nicotinic acid and m-inositol were not essential and were excluded from the revised R-2 medium. Growth was better with ammonium and nitrate together than with nitrate as the sole nitrogen source. Cell growth in the R-2 medium was superior to that obtained in B5, Heller, Murashige-Skoog and White media.