The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)

Daniela S. Gerhard(National Institutes of Health), Lukas Wagner(National Institutes of Health), Elise A. Feingold(National Institutes of Health), Carolyn M. Shenmen(National Institutes of Health), Lynette Grouse(National Institutes of Health), Greg Schuler(National Institutes of Health), Steven L. Klein(National Institutes of Health), Susan Old(National Institutes of Health), Rebekah S. Rasooly(National Institutes of Health), Peter J. Good(National Institutes of Health), Mark S. Guyer(National Institutes of Health), Allison M. Peck(National Institutes of Health), Jeffery G. Derge(Science Applications International Corporation (United States)), David J. Lipman(National Institutes of Health), Francis S. Collins(National Institutes of Health), Wonhee Jang(National Institutes of Health), Steven Sherry(National Institutes of Health), Mike Feolo(National Institutes of Health), Leonie Misquitta(National Institutes of Health), Eduardo Lee(National Institutes of Health), Kirill E. Rotmistrovsky(National Institutes of Health), Susan F. Greenhut(National Institutes of Health), Carl F. Schaefer(National Institutes of Health), Kenneth H. Buetow(National Institutes of Health), Tom I. Bonner(National Institutes of Health), David Haussler(University of California, Santa Cruz), Jim Kent(University of California, Santa Cruz), Mark Kiekhaus(University of California, Santa Cruz), Terry Furey(University of California, Santa Cruz), Michael R. Brent(Washington University in St. Louis), Christa Prange(Lawrence Livermore National Laboratory), Kirsten Schreiber(Lawrence Livermore National Laboratory), Nicole Shapiro(Lawrence Livermore National Laboratory), Narayan Bhat(Science Applications International Corporation (United States)), Ralph F. Hopkins(Science Applications International Corporation (United States)), Florence Hsie, Tom Driscoll, Marcelo B. Soares(University of Iowa), T.L. Casavant(University of Iowa), Todd E. Scheetz(University of Iowa), Michael J Brown-stein(University of Iowa), Ted B. Usdin(National Institutes of Health), Toshiyuki Shiraki(National Institutes of Health), Piero Carninci, Yulan Piao, Dawood B. Dudekula(National Institutes of Health), Minoru S.H. Ko(National Institutes of Health), Koichi Kawakami(National Institutes of Health), Yutaka Suzuki(National Institute of Genetics), Sumio Sugano(The University of Tokyo), C. E. Gruber(The University of Tokyo), M. Smith, Blake A. Simmons, Troy Moore, Richard Waterman, Stephen L. Johnson(Washington University in St. Louis), Yijun Ruan(Washington University in St. Louis), Chia Lin Wei(Agency for Science, Technology and Research), Sinnakaruppan Mathavan(Agency for Science, Technology and Research), Preethi H. Gunaratne(Agency for Science, Technology and Research), Jiaqian Wu(Baylor College of Medicine), Angela Garcia(Baylor College of Medicine), Stephen W. Hulyk(Baylor College of Medicine), Edwin Fuh(Baylor College of Medicine), Ye Yuan(Baylor College of Medicine), Anna Sneed(Baylor College of Medicine), Carla Kowis(Baylor College of Medicine), Anne V. Hodgson(Baylor College of Medicine), Donna M. Muzny(Baylor College of Medicine), John D. McPherson(Baylor College of Medicine), Richard A. Gibbs(Baylor College of Medicine), Jessica Fahey(Baylor College of Medicine), Erin Helton(University of Iowa), Mark Ketteman, Anuradha Madan, Stephanie Rodrigues(University of Iowa), Amy Sanchez(University of Iowa), Michelle Whiting, Anup Madari, Alice Young(University of Iowa), Keith Wetherby(National Institutes of Health), Steven J Granite(National Institutes of Health), Peggy N Kwong(National Institutes of Health), Charles P. Brinkley(National Institutes of Health), Russell L Pearson(National Institutes of Health), Gerard G. Bouffard(National Institutes of Health), Robert W Blakesly(National Institutes of Health), Eric D. Green(National Institutes of Health), Mark Dickson(National Institutes of Health), Álex Rodríguez(Stanford University), Jane Grimwood(Stanford University), Jeremy Schmutz(Stanford University), R Myers(Stanford University), Yaron S.N. Butterfield(Stanford University), Malachi Griffith(University of British Columbia), Obi L. Griffith(University of British Columbia), Martin Krzywinski(University of British Columbia), Nancy Liao(University of British Columbia), Ryan Morin(University of British Columbia), Ryan Morrin(University of British Columbia), Diana Palmquist(University of British Columbia), Anca S. Petrescu(University of British Columbia), Ursula Skalska(University of British Columbia), Duane E. Smailus(University of British Columbia), Jeff Stott(University of British Columbia), Angelique Schnerch(University of British Columbia), Jacqueline E. Schein(University of British Columbia), Steven J.M. Jones(University of British Columbia), Robert A. Holt(University of British Columbia), Ágnes Baross(University of British Columbia), Marco A. Marra(University of British Columbia), Sandra W. Clifton(Washington University in St. Louis), Kathryn A. Makowski, Stephanie Bosak, Joel A. Malek
Genome Research
October 15, 2004
10.1101/gr.2596504
Cited by 590Open Access
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Abstract

The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline.

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