A colorimetric method for the determination of <i>N</i>-acetylglucosamine and <i>N</i>-acetylchrondrosamine

Abstract

THE reaction of acetylated glucosamine with p-dimethylaminobenzaldehyde was first observed by Muller [1901] who showed that after warming a solution of penta-acetylglucosamine with dilute potassium hydroxide an intense reddish- purple colour was produced by the addition of a solution of p-dimethylamino- benzaldehyde acidified with hydrochloric acid [Ehrlich's reagent; 1901].