Characterization of a Nuclease Produced by Staphylococcus aureus
James N. Heins(National Institutes of Health), Juan Suriano(National Institutes of Health), Hiroshi Taniuchi(National Institute of Arthritis and Musculoskeletal and Skin Diseases), Christian B. Anfinsen(National Institute of Arthritis and Musculoskeletal and Skin Diseases)
Cited by 135Open Access
Abstract
The conformational parameters of a nuclease produced by growing cultures of Staphylococcus aureus have been determined. This nuclease exhibits both ribonuclease and deoxyribonuclease activities. It has been shown to have a pH optimum of 9.2 and an absolute requirement for calcium ions. The experimental data are compatible with a compact globular protein of mol wt 17,000 and with a helical content of approximately 45%.
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