A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase
Damien Baud(University College London), Nadine Ladkau(University College London), Thomas S. Moody(Alma Consulting Group), John M. Ward(London Centre for Nanotechnology), H. C. Hailes(University College London)
Cited by 73Open Access
Abstract
A new colorimetric method has been developed to screen transaminases using an inexpensive amine donor. The assay is sensitive, has a low level of background coloration, and can be used to identify and profile transaminase activities against aldehyde and ketone substrates in a high-throughput format. Significantly it is also amendable to solid phase colony screening.
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