High frequency of t(12;21) in childhood B-lineage acute lymphoblastic leukemia
Serge Romana(Institut National de Santé Publique), Healegne Poirel(Institut National de Santé Publique), M Leconiat(Institut National de Santé Publique), MA Flexor(Institut National de Santé Publique), M Mauchauffé(Institut National de Santé Publique), Philippe Jonveaux(Institut National de Santé Publique), EA Macintyre(Institut National de Santé Publique), Roland Berger(Institut National de Santé Publique), Olivier Bernard(Institut National de Santé Publique)
Cited by 442Open Access
Abstract
The recurrent t(12;21)(p12;q22) translocation fuses two genes, TEL and AML1, that have previously been shown to be independently involved in myeloid malignant proliferations. A search for rearrangement of the TEL locus in the region known to be involved in t(12;21) was performed by Southern blotting in a panel of hematopoietic malignancies. The presence of a t(12;21) was confirmed by fluorescence in situ hybridization (FISH) and/or reverse transcriptase (RT)-polymerase chain reaction (PCR). We report that fusion of TEL to AML1 is specifically observed in at least 16% of the childhood B-lineage acute lymphoblastic leukemia (ALL) investigated, none of which had been previously identified as harboring t(12;21).