Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction
Victor M. Corman(University of Bonn), Isabella Eckerle(University of Bonn), Tobias Bleicker(University of Bonn), Ali M. Zaki(Soliman Fakeeh Hospital), Olfert Landt(TIB Molbiol (Germany)), Monika Eschbach-Bludau(University of Bonn), Sander van Boheemen(Erasmus University Rotterdam), Robin Gopal, Matthias Ballhause(TIB Molbiol (Germany)), Theo M. Bestebroer(Erasmus University Rotterdam), Doreen Muth(University of Bonn), Marcel A. Müller(University of Bonn), Jan Felix Drexler(University of Bonn), Maria Zambon, Albert D. M. E. Osterhaus(Erasmus University Rotterdam), Ron A. M. Fouchier(Erasmus University Rotterdam), Christian Drosten(University of Bonn)
Cited by 581Open Access
Abstract
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.
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