An Adenosine 3′,5′-Monophosphate-dependant Protein Kinase from Rabbit Skeletal Muscle

Abstract

Abstract A protein kinase that catalyzes an adenosine 3',5'-monophosphate (cyclic AMP)-dependent phosphorylation of casein and protamine has been purified from rabbit skeletal muscle. The Km values of cyclic AMP for these reactions are 1 x 10-7 and 6 x 10-8 m, respectively. The protein kinase markedly increases the rate of the cyclic AMP-dependent activation and phosphorylation of phosphorylase kinase by ATP.