Effect of internal viral sequences on the utility of retroviral vectors
Donna Armentano(Princeton University), S F Yu(Princeton University), Philip W. Kantoff(Princeton University), T von Rüden, W. French Anderson, Eli Gilboa
Cited by 345Open Access
Abstract
Expression of the human ADA cDNA encoded by the Moloney murine leukemia virus spliced RNA form is enhanced by intron-contained sequences. The presence of sequences corresponding to the viral gag gene in a Moloney murine leukemia virus-based vector results in the generation of 10- to 40-fold higher titers of virus.