Peptide Separation by Two-dimensional Chromatography and Electrophoresis

Abstract

The separation of peptides from controlled proteolytic digests by two-dimensional paper chromatography and electrophoresis is finding wide application to studies of protein structure. With this method it is possible to compare digestion mixtures from different proteins and to detect differences as slight as the replacement of a single amino acid. Such differences are of importance in the comparison of homologous proteins from different animal and plant species, and in the study of genetically determined changes in protein structure, such as those resulting from a single gene mutation.