Guide to molecular cloning techniques.

Abstract

This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Available in an easy-to-use spiral-bound edition, Guide to Molecular Cloning Techniques progresses from basic techniques underlying much of recombinant DNA technology to a series of sections addressing commonly met problems. Comprehensive protocols are compiled specifically for this volume. Among the topics covered are: Genomic cloning; Preparation and characterization of mRNA cDNA cloning; Screening libraries; Identification and characterization of clones; Methods written by 87 experts; Editors' notes, overviews, and extensive cross-referencing. It includes a Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility. It adopts a problem-solving quantitative approach. It places emphasis on strategies for choosing alternative methods.