DNA Fingerprinting in Plants and Fungi

Abstract

Genetic Variation at the DNA Level. A Short Survey of Molecular Markers. Minisatellites and Simple Sequences: The Molecular Basis of Classical DNA Fingerprinting. Detection of DNA Polymorphisms by PCR-Based Fingerprinting. Laboratory Equipment. Methodology. Safety Precautions. DNA Isolation and Purification. DNA Fingerprinting Based on Hybridization. PCR-Based DNA Fingerprinting. Evaluation of Fragment Patterns. Applications of DNA Fingerprinting in Plants and Fungi. Development of Methods and Initial Investigations. Wild Plant Species. Cultivated Plant Species. Inheritance of DNA Fingerprints, Linkage Analysis and Genome Mapping in Plants. Fungi: Human Pathogens, Plant Pathogens, Insect Pathogens, Non-Pathogenic Fungi, and Commercially Important Fungi. Conclusions. Comparison of Methods for Detecting Genetic Variation. Morphological Characters and Allozymes vs. DNA Markers. Different Kinds of Molecular Markers. Future Prospects. Improvement of Existing Met hods. Extending the Spectrum of DNA Typing Strategies. References. Appendices. Index.