Manipulating the Mouse Embryo: A Laboratory Manual

Abstract

Genetics and embryology of the mouse: past, present, and future -- Summary of mouse development -- A mouse colony for the production of transgenic and chimeric animals -- Recovery and in vitro culture of preimplantation embryos -- Isolation, culture, and manipulation of postimplantation embryos -- Surgical procedures -- Production of transgenic mice by pronuclear microinjection -- Embryo-derived stem cell lines -- Germ line-competent stem cells derived from adult mice -- Vector designs for pluripotent stem cell-based transgenesis and genome alterations -- Introduction of foreign DNA into embryonic stem cells -- Production of chimeras -- Genotyping -- Parthenogenesis, pronuclear transfer, and mouse cloning -- Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection -- Cryopreservation, rederivation, and transport of mouse strains -- Techniques for visualizing gene products, cells, tissues, and organ systems -- Setting up a micromanipulation lab