Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay
Andrew M. Kropinski(Public Health Agency of Canada), Amanda Mazzocco, Thomas E. Waddell, Erika J. Lingohr(Public Health Agency of Canada), Roger P. Johnson(Public Health Agency of Canada)
Cited by 1,075
Abstract
The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.
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