Biao Liu

An increasing number of TFE3 rearrangement-associated tumors, such as TFE3 rearrangement-associated perivascular epithelioid cell tumors (PEComas), melanotic Xp11 translocation renal cancers, and melanotic Xp11 neoplasms, have recently been reported. We examined 12 such cases, including 5 TFE3 rearrangement-associated PEComas located in the pancreas, cervix, or pelvis and 7 melanotic Xp11 translocation renal cancers, using clinicopathologic, immunohistochemical, and molecular analyses. All the tumors shared a similar morphology, including a purely nested or sheet-like architecture separated by a delicate vascular network, purely epithelioid cells displaying a clear or granular eosinophilic cytoplasm, a lack of papillary structures and spindle cell or fat components, uniform round or oval nuclei containing small visible nucleoli, and, in most cases (11/12), melanin pigmentation. The levels of mitotic activity and necrosis varied. All 12 cases displayed moderately (2+) or strongly (3+) positive immunoreactivity for TFE3 and cathepsin K. One case labeled focally for HMB45 and Melan-A, whereas the others typically labeled moderately (2+) or strongly (3+) for 1 of these markers. None of the cases were immunoreactive for smooth muscle actin, desmin, CKpan, S100, or PAX8. PSF-TFE3 fusion genes were confirmed by reverse transcription polymerase chain reaction in cases (7/7) in which a novel PSF-TFE3 fusion point was identified. All of the cases displayed TFE3 rearrangement associated with Xp11 translocation. Furthermore, we developed a PSF-TFE3 fusion fluorescence in situ hybridization assay for the detection of the PSF-TFE3 fusion gene and detected it in all 12 cases. Clinical follow-up data were available for 7 patients. Three patients died, and 2 patients (cases 1 and 3) remained alive with no evidence of disease after initial resection. Case 2 experienced recurrence and remained alive with disease. Case 5, a recent case, remained alive with extensive abdominal cavity metastases. Our data suggest that these tumors belong to a single clinicopathologic spectrum and expand the known characteristics of TFE3 rearrangement-associated tumors.

Urban morphology quantitatively expresses a city’s spatial structure, internal relationships, and physical form. It has advantages for predicting urban growth and analyzing the current state of cities in the literature. A comprehensive study on the complex relationships between urban morphology and urban heat island intensity (UHII) is of great importance for mitigating the urban heat island (UHI) effect for megacities. This study models urban morphological indicators in fine resolution based on three aspects: building morphology, ecological infrastructure, and human activities. The model accurately captures UHII by employing the definition of UHI effects. The relationship between urban morphology and UHII was further examined using extreme gradient boosting (XGBoost) and Shapley additive explanations (SHAP). By taking central Beijing, China as study area, major findings include the following: (1) Significant daytime UHI effects were observed within the research area, particularly during the summer months, when it appears to be most severe. More than 90% of the region experiences varying degrees of the UHI effects. (2) UHI is significantly correlated with both 2D and 3D urban morphological indicators. Low sky view factor (SVF) and high SVF tend to mitigate UHI, whereas moderate SVF tends to aggravate UHI. (3) In densely populated areas, tall trees may be more effective than other forms of vegetation at mitigating UHI. Based on the aforementioned findings, this article suggests that urban morphology optimization should focus on seasonality, spatial specificity, and indicator specificity for megacities in urban design and spatial planning aimed at mitigating UHI.

Stem cell transplantation has emerged as a promising avenue in regenerative medicine, potentially facilitating tissue repair in degenerative diseases and injuries. This review comprehensively examines recent developments and challenges in stem cell transplantation. It explores the identification and isolation of various stem cell types, including embryonic, induced pluripotent, and adult stem cells derived from multiple sources. Additionally, the review highlights the tissue-specific applications of these stem cells, focusing on bone and cartilage regeneration, treatment of neurological disorders, and management of hematological conditions. Future advancements and effective resolution of current challenges will be crucial in fully realizing the potential of stem cell transplantation in regenerative medicine. With responsible and ethical practices, the field can potentially transform disease and injury treatment, ultimately improving the quality of life for countless individuals.

These findings implicated Sufu as an important inhibitor of TRAF6 in sepsis and suggest that therapeutics targeting Sufu-TRAF6 may greatly benefit the treatment of sepsis.