Zhe Zhang

Fluorescence imaging in the second near-infrared window (NIR-II) allows visualization of deep anatomical features with an unprecedented degree of clarity. NIR-II fluorophores draw from a broad spectrum of materials spanning semiconducting nanomaterials to organic molecular dyes, yet unfortunately all water-soluble organic molecules with >1,000 nm emission suffer from low quantum yields that have limited temporal resolution and penetration depth. Here, we report tailoring the supramolecular assemblies of protein complexes with a sulfonated NIR-II organic dye (CH-4T) to produce a brilliant 110-fold increase in fluorescence, resulting in the highest quantum yield molecular fluorophore thus far. The bright molecular complex allowed for the fastest video-rate imaging in the second NIR window with ∼50-fold reduced exposure times at a fast 50 frames-per-second (FPS) capable of resolving mouse cardiac cycles. In addition, we demonstrate that the NIR-II molecular complexes are superior to clinically approved ICG for lymph node imaging deep within the mouse body.

Fluorescence probes in the NIR-IIa region show drastically improved imaging owing to the reduced photon scattering and autofluorescence in biological tissues. Now, NIR-IIa polymer dots (Pdots) are developed with a dual fluorescence enhancement mechanism. First, the aggregation induced emission of phenothiazine was used to reduce the nonradiative decay pathways of the polymers in condensed states. Second, fluorescence quenching was minimized by different levels of steric hindrance to further boost the fluorescence. The resulting Pdots displayed a fluorescence QY of ca. 1.7 % in aqueous solution, suggesting an enhancement of ca. 21 times in comparison with the original polymer in tetrahydrofuran (THF) solution. Small-animal imaging by using the NIR-IIa Pdots exhibited a remarkable improvement in penetration depth and signal to background ratio, as confirmed by through-skull and through-scalp fluorescent imaging of the cerebral vasculature of live mice.

Significance On-skin electronics are usually fabricated by patterning conventional inorganic materials, novel organic materials, or emerging nanomaterials on flexible polymer substrates. Consequently, the state-of-the-art on-skin electronics usually suffer from expensive precursor materials, costly fabrication facilities, complex fabrication processes, and limited disposability. By using widely accessible pencils and papers as tools, we have developed a variety of cost-effective and disposable on-skin electronic devices, ranging from biophysical sensors and sweat biochemical sensors to thermal stimulators, humidity energy harvesters, and transdermal drug-delivery systems. Also, pencil–paper-based antennas, two-dimensional and three-dimensional circuits, and reconfigurable structures are demonstrated. The enabled devices can find wide applications particularly in low-resource environments and home-centered personal healthcare owing to their low-cost resources, handy operation, time-saving fabrication, and abundant potential designs.