David E. Minnikin, Anthony G. O’Donnell, Michael Goodfellow et al.|Journal of Microbiological Methods|1984
Cited by 5.5k
Murdoch University
ORCID: 0000-0003-2001-4533Publishes on Soil Carbon and Nitrogen Dynamics, Microbial Community Ecology and Physiology, Genomics and Phylogenetic Studies. 166 papers and 19.2k citations.
Add your photo, update your bio, and get notified when your ranking changes.
A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction. Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.
Partial table of contents: Chemosystematics: Current State and Future Prospects (M. Goodfellow & A. O'Donnell). Analysis of Cellular Constituents from Gram-Negative Bacteria (E. Jantzen & K. Bryn). Analysis of the Cell Wall Constituents of Gram-Positive Bacteria (I. Hancock). Structural Lipids of Eubacteria (T. Embley & R. Wait). Analysis of Microbial Sterols and Hopanoids (G. Jones, et al.). Archaeal Lipids (M. De Rosa & A. Gambacorta). Isoprenoid Quinones (M. Collins). Analysis of Cytochromes (R. Poole). Analysis of Pigments: Carotenoids and Related Polyenes (K. Schmidt, et al.). Determination of DNA Base Composition (J. Tamaoka). Analytical Fingerprinting Methods (J. Magee). Appendix. Index.