Garima Pandey

Abstract Background The technical innovations are based on the principles of science with the assurance of outweighing their cost and risk factors with the benefits to society. But sometimes, the innovation either itself becomes a risk or brings in some risk factors along with it. For most of the alleyway of an innovation from its emergence to its road to societal acceptance and adoption, the focus remains on the benefits majorly. Only when we are at the neck of the hour we think about some of the apparent cost and risk issues. The understanding, proper communication, and address of the basics of risk factors are necessarily required much in advance to deal with this issue. Main body Nanoparticles with very small size and huge surface area are being derived from various plants, microbes, chemical compounds, metals, and metal alloys. Without our realizations, nanotechnology has become a vital part of our day-to-day life, and nanoparticles are proving their worth in almost every field ranging from food, water, medicine, agriculture, construction, fashion, electronics, and computers to eco-remediation, but what about the costs involved and the risks associated? We strongly need to recognize these concerns and challenges, and it requires collaborative efforts from academicians, researchers, industries, government, and non-government organizations to involve people in dialogs to deal with them. Conclusion Through reviewing various studies and articles on nanotechnology, this review has shown that nanotechnology can productively be used to produce consumer goods for pharma, electronics, food, agriculture, aviation, construction, security, and remediation sectors which are advantages in their characteristics. Regarding the future of nanotechnology, we need to focus on assessment and management of risks associated for its promising market growth. Graphical abstract

BACKGROUND: The quality of herbal medicine is determined by its secondary metabolites, which may vary according to growth, season and altitude etc. OBJECTIVE: We studied the variation in phytochemistry and biological activities of Thalictrum foliolosum (TF) roots collected from four sites at different altitudes. MATERIAL AND METHODS: The berberine content in different extracts of T. foliolosum roots collected from various altitudes was estimated using HPTLC. Total phenolic and flavonoid contents were determined using Folin-Ciocalteau reagent and aluminum chloride method respectively. The sensitivity of microbes for the extracts was studied using disc diffusion and the MIC was estimated using broth dilution method. Antioxidant capacity of the plant was studied using β-carotene bleaching assay, lipid peroxidation assay using goat liver, reducing power assay and DPPH free radical scavenging activity. RESULTS: Berberine content varied inversely with altitude; while phenol and flavonoid content of TF increased at higher altitudes. All the TF extracts showed moderate to high activity against Candida albicans, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Extracts with high berberine content were most effective against C. albicans and S. aureus and also showed relatively significant anti-lipid peroxidation, β-carotene bleaching and reducing power. TF extracts with higher phenol and flavonoid content showed better scavenging of DPPH free radicals. Berberine was used as a standard in all the antioxidant and antimicrobial experiments performed. CONCLUSION: Thalictrum from lower elevations can be explored as an alternate source of berberine and the plant has high antioxidant and antimicrobial properties owing to its berberine content.

Berberis is an important genus and well known in the Indian as well as European systems of traditional medicine. It is used since ancient times for curing eye disease, fever, jaundice, rheumatism, vomiting during pregnancy, kidney and gall balder stones and various other ailments due to the presence of biologically active alkaloid berberine. Action of the root extracts of few species are believed to be as powerful as quinine in the treatment of malarial fever. A plethora of literature pertaining to the taxonomy, biology, chemistry, traditional and ethnic uses of Berberis in different countries and indigenous cultures was collected by both offline (library, journals, textbooks etc.) and online mode (electronic search of available databases). In addition to this, books on traditional medicine and ethno pharmacological knowledge were also referred to extract ancient uses of Berberis in different traditional medicine systems. Most of the folklore, traditional and ethno botanical claims about Berberis species were validated by broad spectrum in vitro and vivo pharmacological studies. The present article summarizes its usage in eye and liver disorder, fever, kidney and gall stones along with anticancer activity. This comprehensive review will not only help researchers for further evaluation but also provide substantial information for future exploitation of species to develop novel herbal formulations.

Results: The total phenol, flavonoid and tannin content were estimated to be 1.03%, 5.33% and 1.83% respectively. HPTLC studies revealed the presence of β-sitosterol, lupeol, rutin, ellagic acid, ferulic acid and quercetin in 50% ethanolic extract. The extract showed significant free radical scavenging activity with an IC50 of 110.36μg/ml. Ascorbic acid was taken as the standard antioxidant and its IC50 value was 42 μg/ml. The extract showed significant antibacterial activity against E. coli and S. aureus, though it inhibited the growth of S. aureus more effectively as compared to E. coli. Conclusion: The results are suggestive that leaves of A. indica possess significant antioxidant and antibacterial properties, and contain phytoconstituents that may contribute to its medicinal properties.