Sergey M. Borisov

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOptical BiosensorsSergey M. Borisov and Otto S. WolfbeisView Author Information Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93040 Regensburg, Germany Cite this: Chem. Rev. 2008, 108, 2, 423–461Publication Date (Web):January 30, 2008Publication History Received2 April 2007Published online30 January 2008Published inissue 1 February 2008https://pubs.acs.org/doi/10.1021/cr068105thttps://doi.org/10.1021/cr068105tresearch-articleACS PublicationsCopyright © 2008 American Chemical SocietyRequest reuse permissionsArticle Views19545Altmetric-Citations876LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biotechnology,Carbohydrates,Fluorescence,Peptides and proteins,Sensors Get e-Alerts

values, on the selectivity, sensitivity, precision, dynamic ranges, and temperature dependence of such sensors. Commonly used optical sensing schemes are covered in a next main chapter, with subsections on methods based on absorptiometry, reflectometry, luminescence, refractive index, surface plasmon resonance, photonic crystals, turbidity, mechanical displacement, interferometry, and solvatochromism. This is followed by sections on absorptiometric and luminescent molecular probes for use pH in sensors. Further large sections cover polymeric hosts and supports, and methods for immobilization of indicator dyes. Further and more specific sections summarize the state of the art in materials with dual functionality (indicator and host), nanomaterials, sensors based on upconversion and 2-photon absorption, multiparameter sensors, imaging, and sensors for extreme pH values. A chapter on the many sensing formats has subsections on planar, fiber optic, evanescent wave, refractive index, surface plasmon resonance and holography based sensor designs, and on distributed sensing. Another section summarizes selected applications in areas, such as medicine, biology, oceanography, bioprocess monitoring, corrosion studies, on the use of pH sensors as transducers in biosensors and chemical sensors, and their integration into flow-injection analyzers, microfluidic devices, and lab-on-a-chip systems. An extra section is devoted to current challenges, with subsections on challenges of general nature and those of specific nature. A concluding section gives an outlook on potential future trends and perspectives.

Continuous monitoring of oxygen concentration is of great importance in many different areas of research which range from medical applications to food packaging. In the last three decades, significant progress has been made in the field of optical sensing technology and this review will highlight the one inherent to the development of oxygen indicators. The first section outlines the bioanalytical fields in which optical oxygen sensors have been applied. The second section gives the reader a comprehensive summary of the existing oxygen indicators with a critical highlight on their photophysical and sensing properties. Altogether, this review is meant to give the potential user a guide to select the most suitable oxygen indicator for the particular application of interest.

A simple, high resolution colormetric planar optode imaging approach is presented. The approach is simple and inexpensive yet versatile, and can be used to study the two‐dimensional distribution and dynamics of a range of analytes. The imaging approach utilizes the inbuilt color filter of standard commercial digital single lens reflex cameras to simultaneously record different colors (red, green, and blue) of luminophore emission light using only one excitation light source. Using the ratio between the intensity of the different colors recorded in a single image analyte concentrations can be calculated. The robustness of the approach is documented by obtaining high resolution data of O 2 and pH distributions in marine sediments using easy synthesizable sensors. The sensors rely on the platinum(II)octaethylporphyrin (PtOEP) and lipophilic 8‐Hydroxy‐1,3,6‐pyrenetrisulfonic acid trisodium (HPTS) salt derivate for O 2 and pH measurements, respectively. The brightness of both indicators is dramatically enhanced by making use of energy transfer from a donor molecule (Macrolex yellow coumarin). Furthermore, the emission from the donor serves as an internal reference for the O 2 sensor. The approach relies on semitransparent sensors, facilitating visual inspection of the sediment behind the sensors during measurements. Software for data acquisition and calibration will be available from the authors, whereas all hardware is available from a range of commercial sources. The total cost of the complete measuring system is approximately $3000 US.

Highly photostable and strongly luminescent europium(III) beta-diketonate complexes are presented that can act as new probes for optical sensing of temperature. They can be excited with the light of a 405-nm LED and possess strong brightnesses. The decay times of the probes contained in a poly(vinyl methyl ketone) film and in poly(tert-butyl styrene) microparticles are highly temperature-dependent between 0 and 70 degrees C. The temperature-sensitive microparticles were dispersed, along with oxygen-sensitive microbeads consisting of a palladium porphyrin oxygen indicator in poly(styrene-co-acrylonitrile), in a thin layer of a hydrogel to give a dually sensing material which is excitable by a single light source. The two emissions can be separated by appropriate optical filters. The response to oxygen and temperature is described by 3D plots, and unbiased values can be obtained for temperature and oxygen, respectively, from the two luminescence signals if refined in an iteration step. The sensing scheme is intended for use in temperature-compensated sensing of oxygen, in contactless sensing of oxygen and temperature in (micro)biological and medical applications, in high-resolution oxygen profiling, and for simultaneous imaging of air pressure and temperature in wind tunnels.